Aruna department of microbiology, wilson college, mumbai, maharashtra, india accepted date. Serratia rubidaea and serratia liquefaciens species can be differentiated based on their ability to ferment l arabinose and decarboxylate ornithine. Serratia rubidaea as contaminant in laboratory environment download download pdf short communication. This case emphasizes the need to consider unusual species of serratia in nosocomial sepsis. Statistical optimization and characterization of prodigiosin from a marine serratia rubidaea ramalex. An outbreak of bacteremia was caused by pooling the residual contents of preservative free epoetin vials for later use. Serratia rubidaea definition of serratia rubidaea by. May 3, 2014 serratia marcescens colonies on macconkey agar 1. Apr 12, 2019 a new thermophilic noninduced lipase producer named serratia rubidaea strain nehalmou was isolated from oil waste in tissemsilat, algeria. Biochemical characterization of serratia liquefaciens and. The natural susceptibility to 71 antibiotics of 104 serratia strains of serratia ficaria n 15, serratia fonticola n 18, serratia odorifera n 16, serratia plymuthica n 32 and serratia rubidaea n 23 was examined. Pdf bacterial contamination of fresh tomato fruits is of great concern. Based on phosphate solubilization efficiency and biochemical characterization, we selected one isolate named ed1. Quinoa is renowned for its nutritional value and ability to withstand harsh environmental conditions such as salinity.
Mics were determined using a microdilution procedure in isosensitest broth for all the strains and in cation. Isolation and characterization of a plant growthpromoting. In the early part of the 20 th century, serratia marcescens was considered a nonpathogenic organism and was used in medical experiments and as a biological warfare test agent. The midline catheter was removed, and the patient was successfully treated with intravenous ceftazidime with clinical and bacteriological resolution. Science and technology, general mathematical optimization usage nocardia corallina physiological aspects optimization theory pigments industry pyrrole pyrroles. Figure 1 illustrates the phylogenetic tree showing the most related species to the strain. Serratia rubidaea definition of serratia rubidaea by the.
Serratia marinorubra zobell and upham 1944 approved lists. Serratia rubidaea synonyms, serratia rubidaea antonyms. The silastic sponge creates a new type of environment for microorganisms. Compared with a noninoculated control, the radicular root. Two novel lipids rubiwettins r1 and rg1 were isolated and shown to be able to lower the surface tension of saline to 26 mnm. Statistical optimization and characterization of prodigiosin. Serratia marcescens is the most common species of serratia found in hospitals, and the only pathogenic species of serratia, except for rare reports of diseases resulting from infection with serratia plymuthica, serratia liquefaciens, serratia rubidaea, and serratia odorifera. Plant colonizing bacteria can be found in the rhizosphere as free.
Isolation and characterization of serratia rubidaea from. The geniii gramnegative aerobic bacteria system from biolog can also identify s. In this study, we identified a strain of serratia marcescens that has. The most influential lipase production parameters were screened by the plackettburman design for enhancing enzyme yield. Serratia rubidaea is an opportunist pathogenic bacterium, it is rarely identified in man, and when so, generally found in the respiratory tract, wounds, feces, bile, but also in blood. Halotolerant endophytic bacterium serratia rubidaea ed1. There have been many cases of bacterial contamination in the laboratory.
The sequence was submitted to genbank with accession number km411440 at ncbi. Taxonomy of the species serratia rubidaea stapp 1940 ewing. Although several recent studies disclosed a number of interesting biological properties of prodigiosin, this paper reports a higher value of prodigiosin production by a newly isolated indigenous marine bacteria serratia rubidaea rather than serratia marcescens from a relatively cheap medium. Between june and november 2015, we identified eight patients with suspected bacteremia that produced a positive blood culture for serratia spp. Department of microbiology, melaka manipal medical college, manipal. Isolation and characterization of serratia rubidaea from dark. Taxonomy of the species serratia rubidaea stapp 1940 ewing et al. Tackling intrinsic antibiotic resistance in serratia marcescens with a combination of ampicillinsulbactam and phage salsa.
In view of the potential observed for biosurfactants in the past decade, a number of research activities are presently focused on. Serratia is a genus of gramnegative, facultatively anaerobic, rodshaped bacteria of the family. What are the risk factors for serratia sepsis or bacteremia. They studied the effect of salt stress on pigment production in serratia rubidaea n1 and observed a higherlevel of pigment synthesis at 3. Serratia is found in soil, water, plants, and human intestine. The bacterial genera identified as contaminants are bacillus, staphylococcus, micrococcus, pseudomonas, shigella and serratia. Of these serratia species, serratia marcescens is the one most commonly associated with human infections 16. Pdf serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who.
This isolate has shown capacities for indole acetic acid production and siderophores synthesis. Sy5, was isolated from the rhizoplane of barnyard grass echinochloa crusgalli grown in petroleum and heavymetalcontaminated soil. Mar 08, 2011 isolation and characterization of serratia rubidaea from dark brown spots of tomato fruits isolation and characterization of serratia rubidaea from dark brown spots of tomato fruits abdalla, mohamed. A chest xray demonstrated a new right upper lobe infiltrate, and the blood culture grew serratia rubidaea. Serratia rubidaea as contaminant in laboratory environment. Within the enterobacteriaceae, strains of the genus serratia are frequently encountered in human. Currently, four species of serratia have been found in sea water. Strains of the genus serratia as beneficial rhizobacteria of oilseed. Plantassociated serratia consists of both endophytic and free living species in. Serratia plymuthica an overview sciencedirect topics.
In the early part of the 20 th century, serratia marcescens was considered a nonpathogenic organism and was used in medical experiments and as a biological warfare test agent 1. The isolation of this organism from clinical specimens is rare,10,11 but it can cause opportunistic infections in severely ill patients receiving. Serratia marinorubra zobell and upham 1944 approved lists 1980. Serratia liquefaciens bloodstream infections from contamination of epoetin alfa at a hemodialysis center.
Pdf role of pigment in the stress response of serratia. Millerkeane encyclopedia and dictionary of medicine, nursing. The infection was cleared after adequate treatment with antibiotics. Serratia marcescens secretes proteases and chitinases. Lactamase producers and its enviromental application anuradha pendse, aruna k adjunct professor, microbiology department, wilson college, mumbai, india abstract.
In this study we isolated phytopathogenic serratia rubidaea from imported in bulgaria diseased holland tulip bulbs. Surfaceactive novel glycolipid and linked 3hydroxy fatty. The strain serratia rubidaea snau02 was isolated from the hydrocarbon contaminated soils of cuddalore district, tamilnadu, india. A study was made of the biochemical reactions given by 109 cultures of enterobacter liquefaciens and 49 strains of serratia rubidaea. Biochemical characterization of serratia liquefaciens grimes and hennerty bascomb et al. Serratia rubidaea rhizobacteria of oilseed rape antifungal mechanisms.
Tween 80 medium for differentiating nonpigmented serratia. Serratia bizio 1823 approved lists 1980 assigned by. Members of the genus serratia, particularly the type species serratia marcescens, cause important infections in humans, animals, and insects. Feb 27, 2015 form fda 3881 814 page 1 of 5 psc publishing services 301 4436740 ef department of health and human services food and drug administration indications for use form approved. Serratia species isolated from bovine intramammary infections1. Role of pigment in the stress response of serratia rubidaea isolated from annona reticulata. Serratia rubidaea synonyms, serratia rubidaea pronunciation, serratia rubidaea translation, english dictionary definition of serratia rubidaea. The bacteria produce a red pigment which is easily seen when grown on bloodfree media. Production, extraction and characterization of red pigment. Serratia species isolated from bovine lntramammary infections.
Zinc release from zno, zn3po42, or znco3 revealed the ef. Feb 20, 2019 serratia bacteria species are opportunistic gramnegative bacteria classified in the tribe klebsielleae and the large family enterobacteriaceae and comprises at least 14 species and two subspecies. Serratia rubidaea, although rarely recovered from human specimens, is recognized as the fourth common cause of serratia related infections. Taxonomically, the genus serratia is confusing, and currently there are 14 recognized species, with 2 subspecies, in the genus table 1. Summary serratia species, in particular serratia marcescens, are significant human pathogens. Production of extracellular lipase in submerged culture of serratia rubidaea has been investigated. International journal of systematic bacteriology 1973. Serratia rubidaea is a potential strain for lipase production by utilizing some of the cheapest substrates.
Serratia rubidaea as contaminant in laboratory environment download download pdf. The gramnegative bacterial genus serratia is found within the broad enterobacteriaceae family and is currently differentiated into 10 species. Antibiotics free fulltext tackling intrinsic antibiotic resistance in. A new thermophilic noninduced lipase producer named serratia rubidaea strain nehalmou was isolated from oil waste in tissemsilat, algeria. Outbreaks of serratia marcescens and serratia rubidaea. Similar patterns of in the active culture medium containing 0. Investigation of lipase production by milk isolate serratia rubidaea. In the present work, we isolated 34 phosphate solubilizing endophytic bacteria associated with the roots of quinoa plants. Draft genome sequence of the serratia rubidaea cip 103234t. Pdf serratias are widely distributed in soil, air and water, plantand animal associated bacteria. Serratia ficaria, an enterobacterium involved in the fig tree ecosystem, has been isolated from human clinical samples in rare instances, and its role as a pathogen is unclear. Gramnegative bacilli growth bothaerobicandanaerobic catalasepositive oxidasenegative indolenegative ureasenegative tsi no production of h2s vancomycinresistant colistineresistant. Pdf serratias are widely distributed in soil, air and water, plantand animalassociated bacteria. The characterization of morphological and biochemical characters of the isolate serratia rubidaea snau02 was carried out according to the berges manual of determinative bacteriology.
Paraplegia could be a predisposing factor for this organism to cause urinary tract infection. Serratia rubidaea biotype 1 was isolated from the bile and blood of a patient with a bile tract carcinoma obstructing the common bile duct and who underwent invasive procedures. Rna gene analysis revealed that the selected isolate shares 99. The bacterium caused a red discolouration of the sponge and the surrounding part of the leg. Serratia marcescens bacteria, sources of infection, risk. Various strains of serratia occupy an eclectic range of habitats. Rarely,it has been found in blood,the respiratory tract, wounds. Plant growth promoting pgp studies showed that the ed1 strain solubilized complexed forms of phosphate ca3po42.
Serratia rubidaea is a less welldescribed member of the genus and is chie. Serratia rubidaea isolated from a silastic foam dressing. Clinical significance rarely from clinical material. A plant growthpromoting rhizobacterium, serratia sp. These lipids were located in extracellular vesicles found in a 30 degrees c culture of s. Pdf isolation and characterization of serratia rubidaea from dark. Outbreaks of serratia marcescens and serratia rubidaea bacteremia. The subspecies burdigalensis,colindalensis and rubidaea of serratia rubidaea were created in 1990 by grimont et al. Production optimization, characterization, and covalent. Lps was extracted from serratia rubidaea and detected by the addition of schiffs reagent. Serratia species that can be identified by the vitek 2 gn card, from biomerieux, are s. Thus, the present study shows that pigment production in serratia rubidaea, a model organism used in the work, is influenced by the factors used in study including temperature of growth, salt. A pigmentproducing strain ofserratia rubidaea was isolated from two consecutive cultures from a sponge of silastic foam used as a dressing for chronic crural ulcers. Lipase production by serratia rubidaea, food technol biotechnol.
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